It seems to me that the question is not whether the file fits into the DVD, but rather if its size fits into the variables used by mkisofs to address files and/or doing space calculations. ("mkisofs" is the command used to actually burn the DVD; k3b is just a front-end to other commands.)
No. mkisofs is the program used to create the image, while cdrecord or growisofs is the one used to burn that image.
